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Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice
Published on: July 29, 2022
The alterations of mouse plasma proteins during septic development
Yan Ren1, Jiandong Wang, Ji Xia
1Beijing Genomics Institute, Chinese Academy of Science, Beijing, China.
Journal of Proteome Research
|May 15, 2007
Summary
Early sepsis control is vital. This study identified 13 sepsis-responsive plasma proteins in mice using proteomics, revealing changes in their N-glycosylation during inflammation.
Area of Science:
- Proteomics
- Immunology
- Biochemistry
Background:
- Sepsis therapy requires early inflammation control.
- Cecal ligation and puncture (CLP) surgery models chronic insult in mice.
- Plasma proteomic changes are key indicators of sepsis development.
Purpose of the Study:
- To identify sepsis-responsive plasma proteins.
- To investigate protein alterations in response to CLP surgery.
- To analyze post-translational modifications, specifically N-glycosylation, in sepsis.
Main Methods:
- Cecal ligation and puncture (CLP) mouse model.
- Plasma protein isolation and albumin depletion.
- Two-dimensional electrophoresis (2-DE) for protein separation.
- Matrix-assisted laser desorption/ionization-time of flight/time of flight (MALDI-TOF/TOF) mass spectrometry for protein identification.
- PNGase F digestion and Western blot analysis for N-glycan assessment.
Main Results:
- Over 30 differential protein spots observed 24 hours post-CLP compared to sham surgery.
- 13 unique sepsis-responsive proteins identified.
- Most identified proteins showed multiple altered spots on 2-DE gels.
- Sepsis-associated proteins largely retained N-glycosylation, but N-glycan structures changed.
Conclusions:
- Plasma proteomic analysis effectively captures sepsis-induced changes.
- Specific proteins are identified as potential biomarkers for sepsis.
- Altered N-glycosylation patterns are a significant feature of sepsis progression.
