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Related Experiment Video

Updated: Jul 15, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

Double filtration plasmapheresis can decrease factor XIII Activity.

Norio Hanafusa¹, Yasushi Kondo, Makoto Suzuki

¹Department of Hemodialysis and Apheresis, The University of Tokyo Hospital, Tokyo, Japan. hanafusa-tky@umin.ac.jp

Therapeutic Apheresis and Dialysis : Official Peer-Reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy

|May 15, 2007

Summary

Intense double filtration plasmapheresis (DFPP) significantly reduces Factor XIII (FXIII) activity, potentially impacting wound healing. Monitoring FXIII levels during DFPP is crucial, especially with albumin replacement, to prevent complications.

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Area of Science:

Hematology
Nephrology
Critical Care Medicine

Background:

Factor XIII (FXIII) is essential for stabilizing fibrin clots and ensuring proper wound healing.
Deficiency in FXIII can lead to bleeding disorders and impaired healing.
Double filtration plasmapheresis (DFPP) is a procedure used to remove substances from the blood.

Observation:

Five patients undergoing intense DFPP showed a significant decrease in FXIII activity, often below 10% of baseline.
Standard coagulation tests did not adequately reflect the diminished FXIII levels.
FXIII's properties (molecular weight, half-life, distribution volume) make it susceptible to removal by DFPP.

Findings:

Intense DFPP therapy is associated with a profound reduction in Factor XIII activity.
The decrease in FXIII activity caused by DFPP was clinically observed in the treated patients.
Despite low FXIII levels, fatal bleeding was avoided, though one patient required intervention for prolonged bleeding.

Implications:

Patients undergoing DFPP, particularly intensified regimens with albumin replacement, require monitoring of FXIII activity.
Routine coagulation tests are insufficient to assess FXIII levels during DFPP.
Proactive measurement and potential supplementation of FXIII may be necessary to mitigate bleeding risks in patients receiving DFPP.