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[Effects of PBDE-47 on oxidative stress and apoptosis in SH-SY5Y cell]
Ming Zhang1, Wei-hong He, Ping He
1Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
Objective:
To investigate the oxidative stress and apoptosis induced by 2, 2', 4, 4'-polybrominated diphenyl ethers (PBDE-47) in human neuroblastoma SH-SY5Y cells, and to explore the involved role of reactive oxygen species (ROS) on apoptosis.
Methods:
The rate of cellular survivors, intracellular ROS level, malondialdehyde (MDA) content and percentage of apoptosis were measured respectively after the SH-SY5Y cell were exposed to 2, 4, 8 microg/ml PBDE-47 for 24 h in vitro.
Results:
The rate of cellular survivors in the low dose PBDE-47-treated group was higher than the control group (P < 0.05), but the moderate and high dose PBDE-47-treated groups were significantly lower than the control group (P < 0.05). The MDA contents in the moderate and high dose PBDE-47-treated groups were significantly higher than the control group (P < 0.05) and increased with increase of PBDE-47 exposure concentrations. Compared with the control group, the levels of ROS were significantly increased with increase of PBDE-47 concentrations (P < 0.05). In the moderate and high dose PBDE-47-treated groups, the percentages of apoptosis were significantly higher than that of the control group (P < 0.05).
Conclusion:
PBDE-47 can induce oxidative stress and apoptosis in SH-SY5Y cell. ROS may play an important role in the apoptosis induced by PBDE-47.
Insights
Polybrominated diphenyl ether-47 (PBDE-47) exposure induces oxidative stress and apoptosis in human neuroblastoma cells. Reactive oxygen species (ROS) play a key role in PBDE-47-induced cell death.
Area of Science:
- Environmental toxicology
- Cell biology
- Neuroscience
Context:
- Polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants found globally.
- PBDE-47 is a common congener with potential neurotoxic effects.
- Human neuroblastoma SH-SY5Y cells provide a relevant in vitro model for studying neurotoxicity.
Purpose:
- To investigate the impact of PBDE-47 on oxidative stress and apoptosis in SH-SY5Y cells.
- To elucidate the role of reactive oxygen species (ROS) in PBDE-47-induced apoptosis.
Summary:
- SH-SY5Y cells were exposed to varying concentrations of PBDE-47 (2, 4, 8 microg/ml) for 24 hours.
- PBDE-47 exposure led to decreased cell viability, increased malondialdehyde (MDA) levels, and elevated intracellular ROS.
- Apoptosis percentages significantly increased in moderate and high PBDE-47 dose groups.
Impact:
- PBDE-47 induces significant oxidative stress and apoptosis in neuronal cells.
- ROS generation is a critical mediator of PBDE-47 neurotoxicity.
- Findings highlight the potential neurotoxic risks of PBDE-47 exposure.
