Tubby protein in human lymphocytes from normal weight and obese subjects
Gino Giannaccini1, Laura Giusti, Ferruccio Santini
1Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, Environment and Endocrine and Nervous Systems High Technology Center for the Study of the Effects of Harmful Agents, University of Pisa, Via Bonanno 6, Pisa, Italy. ggino@farm.unipi.it
Objectives:
The presence of tubby protein in human lymphocytes was investigated and their electrophoretic mobility property between normal weight and obese subjects was compared.
Design And Methods:
2-DE proteome map of lymphocytes has been generated and western blot analysis was conducted using anti-tub polyclonal antibody.
Results And Conclusions:
We found the presence of tubby protein both in normal weight and in obese subjects; however in the latter an isoelectric point shift toward the acidic end was observed.
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