Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Telomeric nucleosomes are intrinsically mobile.

Sabrina Pisano¹, Enrico Marchioni, Alessandra Galati

¹Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Roma, Italy.

Journal of Molecular Biology

|May 15, 2007

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Distinct patterns of small-fiber dysfunction in anti-myelin-associated glycoprotein-positive and negative paraproteinemic neuropathies.

Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology·2026

Same author

Comparative Study of Sporadic and Immune Checkpoint Inhibitor-Related Forms of Myasthenia Gravis-Myositis Overlap Syndrome.

Neurology·2026

Same author

Addendum: Post-transcriptional gene silencing mediated by microRNAs is controlled by nucleoplasmic Sfpq.

Nature communications·2026

Same author

Multiple sclerosis-associated EBNA2 variants influence the response to peginterferon beta-1a therapy.

Journal of autoimmunity·2026

Same author

Human mammary 3D spheroid models uncover the role of filopodia in breaching the basement membrane to facilitate invasion.

Matrix biology : journal of the International Society for Matrix Biology·2025

Same author

Modulating TERRA G-quadruplexes with ligands: impact on telomeric DNA:RNA hybrids and ALT mechanisms.

Nucleic acids research·2025

Same journal

Biologically Relevant, Cationic Residues in Human Rhinovirus Stabilize Capsid-Bound RNA Duplexes, and Restrict Capsid Flexibility.

Journal of molecular biology·2026

Same journal

Cryo-EM structures of phage T4 infection intermediate.

Journal of molecular biology·2026

Same journal

A classic fold with a twist: Structural architecture of Dhillonvirus phage Bas18.

Journal of molecular biology·2026

Same journal

Tesorai Search: cloud-based database search engine boosts identifications for mass spectrometry proteomics with a pretrained peptide-spectrum deep-learning model.

Journal of molecular biology·2026

Same journal

Characterization of diverse functions of NRF1 nuclear localization sequence.

Journal of molecular biology·2026

Same journal

UPF3A and UPF3B shape the transcriptome cooperatively yet oppose cell function.

Journal of molecular biology·2026

See all related articles

Telomeric nucleosomes exhibit high intrinsic mobility, unlike those on average DNA sequences. DNA sequence significantly influences nucleosome dynamics and repositioning, impacting telomeric chromatin dynamics.

Area of Science:

Molecular Biology
Genetics
Biophysics

Background:

Nucleosomes, the basic units of eukaryotic DNA packaging, are dynamic and crucial for chromosomal processes.
ATP-dependent chromatin remodelers facilitate protein access by sliding nucleosomes.
Spontaneous nucleosome repositioning occurs due to intrinsic dynamics, but sequence-dependent contributions are unclear.

Purpose of the Study:

To investigate the sequence-dependent dynamic properties of nucleosomes.
To determine the contribution of DNA sequence to nucleosome repositioning.
To explore the mobility of nucleosomes on telomeric sequences.

Main Methods:

Studied nucleosome mobility on telomeric and average DNA sequences.
Varied temperature and ionic strength to assess dynamic properties.

Related Experiment Videos

Analyzed nucleosome repositioning under different conditions.

Main Results:

Telomeric nucleosomes display high intrinsic mobility under physiological conditions.
Nucleosomes on average DNA sequences show limited repositioning.
DNA sequence significantly impacts nucleosome thermodynamic stability, positioning, and dynamic properties.

Conclusions:

DNA sequence is a key determinant of nucleosome dynamic properties, not just stability and positioning.
The high intrinsic mobility of telomeric nucleosomes may play a role in telomeric chromatin dynamics.