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Updated: May 2, 2026
Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
Published on: June 24, 2016
Development and validation of a fast method for determination of free glycerol in biodiesel by capillary
Luiz Carlos Gonçalves Filho1, Gustavo Amadeu Micke
1Departamento de Química, Universidade Federal de Santa Catarina, CEP 88040-900, Campus Universitário Trindade, Florianópolis, SC, Brazil.
Abstract:
In this study, a capillary electrophoresis (CE) methodology for the determination of free glycerol in biodiesel using oxidative cleavage with periodate was optimized and validated. The amount of iodate produced in the reaction was determined by CE. The optimized electrolyte was 20 mmol L(-1) glycine and 10 mmol L(-1) trifluoroacetic acid (direct UV detection, 210 nm). The short total analysis time (less than 28 s) was obtained using the short end injection mode. The optimization of the method was carried out using Peakmaster software. The choice of the components of the run electrolyte and of the internal standard (nitrate) was made through the use of effective mobility curves. A good correlation coefficient higher than 0.9991 and low LOD 4.3 mg L(-1) was obtained. The recovery of free glycerol was 95.4-102.4%. This method was used to determine glycerol in commercial biodiesel samples.
