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A nonisotopic assay for unambiguous assignment of DNA glycosylase functionality

Heiko Kuhn1

  • 1Center for Advanced Biotechnology, Department of Biomedical Engineering, Boston University, 36 Cummington St., Boston, MA 02215, USA. hkuhn@bu.edu

Analytical Biochemistry
|May 15, 2007
PubMed
Abstract

No abstract available in PubMed .

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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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