Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Membrane Asymmetry Regulating Transporters

Membrane Asymmetry Regulating Transporters

Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...

Asymmetric Lipid Bilayer

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Membrane Fluidity

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...

Cell Migration

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A simple clinical score to reduce unnecessary testing for Puumala hantavirus.

PloS one·2024

Same author

Reduction of Endothelial Glycocalyx on Peritubular Capillaries in Chronic Kidney Disease.

The American journal of pathology·2022

Same author

Genetic regulation of serum IgA levels and susceptibility to common immune, infectious, kidney, and cardio-metabolic traits.

Nature communications·2022

Same author

The Role of Platelet-Derived Growth Factor in Focal Segmental Glomerulosclerosis.

Journal of the American Society of Nephrology : JASN·2022

Same author

Adult human kidney organoids originate from CD24<sup>+</sup> cells and represent an advanced model for adult polycystic kidney disease.

Nature genetics·2022

Same author

Results from part A of the multi-center, double-blind, randomized, placebo-controlled NefIgArd trial, which evaluated targeted-release formulation of budesonide for the treatment of primary immunoglobulin A nephropathy.

Kidney international·2022

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

Differentially spliced isoforms of FAT1 are asymmetrically distributed within migrating cells.

Gerald S Braun¹, Matthias Kretzler, Torsten Heider

¹Institute for Anatomy and Cell Biology 1, University of Heidelberg, 69120 Heidelberg, Germany.

The Journal of Biological Chemistry

|May 15, 2007

Summary

Wild-type FAT1 drives cell migration and wound healing by localizing to the cell

More Related Videos

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

Immunomagnetic Separation of Fat Depot-specific Sca1^high Adipose-derived Stem Cells (ASCs)

Published on: August 11, 2016

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Published on: March 31, 2018

Related Experiment Videos

Last Updated: Jul 15, 2026

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

Immunomagnetic Separation of Fat Depot-specific Sca1^high Adipose-derived Stem Cells (ASCs)

Published on: August 11, 2016

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Published on: March 31, 2018

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Cadherin FAT1 (FAT1) is crucial for cell polarization and directed migration.
FAT1 maintains the kidney's glomerular filtration barrier structure.
Understanding FAT1's role in cell dynamics is vital for kidney health and cancer research.

Purpose of the Study:

Identify and characterize novel FAT1 splice isoforms.
Investigate the functional and localization differences between FAT1 variants.
Determine the role of FAT1 isoforms in cell migration and tissue repair.

Main Methods:

Identification of novel FAT1 splice variants using molecular techniques.
Subcellular localization studies of FAT1 isoforms via immunofluorescence.
Analysis of FAT1 expression in healthy and diseased kidney tissues.
Functional assays including cell migration, wound healing, and overexpression/knockdown experiments.

Main Results:

Three novel FAT1 splice isoforms (FAT1(+12), FAT1(+32), FAT1(+8TR)) were identified.
Wild-type FAT1 (FAT1(WT)) localizes to the leading edge, while isoforms localize to intercellular junctions.
FAT1(WT) expression increases during cell migration and in glomerulonephritis.
FAT1(WT) promotes cell protrusions and wound healing; isoforms do not.

Conclusions:

FAT1(WT) is the key isoform for cell migration and wound healing.
Alternative splicing of FAT1 alters its subcellular localization and function.
FAT1 isoform dynamics are relevant to kidney disease pathogenesis and cellular repair mechanisms.