Related Experiment Video
Updated: Jul 15, 2026

12:04
Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
Published on: March 27, 2014
A rapid TLC autographic method for the detection of glucosidase inhibitors
Mario O Salazar1, Ricardo L E Furlan
1Catedra de Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
Phytochemical Analysis : PCA
|May 16, 2007
Abstract:
A new bioautographic assay suitable for the localisation of beta-glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex.

