Related Experiment Video
Updated: Jul 14, 2026
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
Published on: November 8, 2016
Expression of Thy 1.2 surface antigen increases significantly during the murine mesenchymal stem cells cultivation
Mohamadreza Baghaban Eslaminejad1, Samad Nadri, Reza Hajji Hosseini
1Stem Cell Department, Royan Institute, PO Box 19395-4644, Tehran, Iran. bagesla@yahoo.com
Abstract:
This study sought to investigate the absence or expression of some surface antigens on murine mesenchymal stem cells (mMSCs) during the cultivation period of primary culture to passage 3 (equivalent to about 15 or 16 population doubling number). For this purpose, bone marrow cells from 6-8-week-old mice (either NMRI or Balb/c) were cultivated in 75-cm(2) culture flask for three successive passages, in each of which the culture was examined for the expression of CD135, CD44, CD31, Thy1.2, CD11b, CD45, CD34, Vcam1, Sca-1, and c-Kit antigens, using flow cytometry. Passage-3 cells from each strain can easily be differentiated into bone and fat, which was indicative of their mesenchymal nature. Our results demonstrated that for each given antigen, the percentages of the cells expressing that antigen had been changed by subcultures. The statistical analysis showed that nearly all differences between the passages were statistically significant. In this term, the expressional changes of Thy 1.2 seemed to be very significant in such a way that the expression increased to about half of the whole population in passage 3. In conclusion, it seems that this antigen could be considered as an enriching antigen for mMSCs population from bone marrow adherent cell culture.
Insights
Surface antigen expression changes in murine mesenchymal stem cells (mMSCs) during culture. Thy 1.2 antigen expression significantly increases by passage 3, suggesting its utility for enriching mMSCs.
Area of Science:
- Cell Biology
- Immunology
- Stem Cell Research
Background:
- Murine mesenchymal stem cells (mMSCs) are crucial for regenerative medicine.
- Characterizing surface antigen expression is vital for understanding stem cell behavior and purity.
Purpose of the Study:
- To investigate the dynamic expression of surface antigens on mMSCs during serial subculturing.
- To identify potential markers for enriching mMSCs populations.
Main Methods:
- Bone marrow-derived cells from mice were cultured for three passages.
- Flow cytometry was used to analyze the expression of ten key surface antigens (CD135, CD44, CD31, Thy1.2, CD11b, CD45, CD34, Vcam1, Sca-1, c-Kit).
- Mesenchymal differentiation potential was confirmed at passage 3.
Main Results:
- Significant alterations in the percentage of cells expressing various antigens were observed across passages.
- Thy 1.2 expression showed a marked increase, reaching approximately 50% of the cell population by passage 3.
- Statistical analysis confirmed the significance of these antigen expression changes.
Conclusions:
- Cultivation significantly impacts surface antigen profiles of mMSCs.
- Thy 1.2 emerges as a promising marker for the enrichment of mMSCs derived from bone marrow adherent cell cultures.
