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Updated: Jul 14, 2026

Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica
Published on: August 23, 2018
Flexibility and strictness in functional replacement of domain III of cry insecticidal proteins from Bacillus
Hiroshi Sakai1, Mohammad Tofazzal Hossain Howlader, Yumiko Ishida
1Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, Okayama-shi, Okayama 700-8530, Japan. sakahrsh@biotech.okayama-u.ac.jp
Abstract:
Cry1C, one of the lepidopteran-specific insecticidal proteins from Bacillus thuringiensis, exhibits potent cytotoxicity against Sf9, an insect cell line. Cry1Aa and Cry4A, which are lepidopteran- and dipteran-specific insecticidal proteins, respectively, show no cytotoxicity against Sf9. When domain III of Cry1C was replaced with that of Cry1Aa or Cry4A, the hybrid Cry1C protein retained the cytotoxicity. These results suggest that domain III of Cry1C is not crucial in determining the cytocidal specificity of Cry1C against Sf9.
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