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Updated: Jan 20, 2026

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
Published on: October 30, 2016
Expression and purification of viral glycoproteins using recombinant vaccinia viruses for functional and structural
Zhu-Nan Li1, David A Steinhauer
1Emory University School of Medicine, Atlanta, GA, USA.
Abstract:
Methods for generating recombinant vaccinia viruses for the expression of foreign viral glycoproteins in mammalian cell lines and the purification of expressed viral glycoproteins are described. These methods are based on many years of experience with the influenza hemagglutinin glycoprotein (HA). However, they are applicable for studies on other viral glycoproteins, and with slight modifications, could be useful for cellular proteins as well.
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