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Published on: July 28, 2023
Structural analysis of expressed metabolic subnetworks
Oliver Ebenhöh1, Wolfram Liebermeister
1Institute for Biology, Humboldt University Berlin, 10115 Berlin, Germany. oliver.ebenhoeh@rz.hu-berlin.de
Summary
Gene regulation in yeast optimizes metabolic networks for available nutrients, enhancing essential compound production while minimizing enzyme costs during the diauxic shift.
Area of Science:
- Cellular metabolism
- Systems biology
- Gene regulation
Background:
- Cellular metabolic capabilities are influenced by enzyme expression, presenting a resource tradeoff.
- Gene regulatory systems must balance metabolic needs with resource consumption.
Purpose of the Study:
- To investigate if gene expression patterns reflect cellular metabolic demands.
- To analyze the metabolic subnetworks expressed during yeast diauxic shift.
Main Methods:
- Translating gene expression profiles into active biochemical reaction sets (metabolic subnetworks).
- Assessing metabolic capacity and essential capacity from various carbon sources.
- Comparing expressed subnetworks during diauxic shift with random selections.
Main Results:
- Metabolic capacities of carbon sources generally decrease during yeast diauxic shift.
- The subnetwork expressed during initial glucose utilization exhibits significantly higher glucose metabolic capacity.
- Essential capacity for glucose production is also maximized in the early glucose-grown subnetwork.
Conclusions:
- Gene regulation enhances the range of essential compounds producible from available nutrients.
- This optimization minimizes the burden of protein synthesis by expressing fewer enzymes.
- Yeast metabolic networks adapt to nutrient availability through strategic gene expression.
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