Related Experiment Video

Updated: Jul 14, 2026

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
08:32

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Published on: March 10, 2017

c-Myc partially mediates IFNgamma-induced apoptosis in the primary hepatocyte

C T McCullough1, B J Tura, D J Harrison

  • 1Edinburgh Cancer Research Centre, University of Edinburgh, Crewe Road South, Edinburgh, UK.

International Journal of Experimental Pathology
|May 17, 2007
PubMed

Insights

Interferon-gamma (IFNgamma) enhances liver cell death by synergizing with apoptotic signals like CD95. This study reveals IFNgamma-induced apoptosis pathways and suggests c-myc

Area of Science:

  • Hepatology
  • Immunology
  • Cell Biology

Background:

  • Interferon-gamma (IFNgamma) is implicated in liver injury during hepatitis.
  • Cytokines and inflammatory responses play a role in hepatocellular damage.

Purpose of the Study:

  • To investigate the role of IFNgamma in primary hepatocyte apoptosis.
  • To elucidate the mechanisms of IFNgamma-mediated liver cell death.
  • To explore potential therapeutic targets for modulating IFNgamma signaling.

Main Methods:

  • Primary hepatocyte culture.
  • Induction of apoptosis using CD95, TNFalpha, and UV irradiation.
  • Assessment of IFNgamma synergy with apoptotic stimuli.
  • Analysis of CD95 and CD95 ligand interactions.
  • Use of c-myc antisense fragments to inhibit apoptosis.

Main Results:

  • IFNgamma synergizes with CD95, TNFalpha, and UV irradiation to induce apoptosis in primary hepatocytes.
  • IFNgamma-induced apoptosis requires CD95 cell surface interaction and induces soluble CD95 ligand release.
  • C-myc antisense fragments suppress IFNgamma-induced hepatocyte apoptosis.
  • Evidence suggests c-myc contributes to IFNgamma signaling.

Conclusions:

  • IFNgamma contributes to hepatocyte apoptosis through distinct pathways involving CD95.
  • Modulation of IFNgamma signaling via cytokines or CD95 interaction interruption can impact hepatocellular response.
  • C-myc plays a role in IFNgamma-mediated cell death pathways.

More Related Videos

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c07:42

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Published on: June 29, 2011

Related Concept Videos

The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Liver Regeneration01:24

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...