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Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Cloning and sequencing of ovine Flt3 ligand
S S M Chan1, N Lyons, I McConnell
1Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, UK.
International Journal of Immunogenetics
|May 17, 2007
Abstract:
A cDNA (879 bp) containing the complete open reading frame of ovine Fms-related tyrosine kinase 3 ligand (Flt3-L) was amplified by reverse transcriptase polymerase chain reaction (RT-PCR), cloned and sequenced. The deduced amino acid sequence has 97.6% similarity with bovine Flt3-L isoform 1 and shares the long cytoplasmic domain observed in bovine Flt3-L but not in human Flt3-L.

