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High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

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Updated: Jul 14, 2026

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

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Triterpenoids from swallow roots--a convenient HPLC method for separation.

S Nagarajan¹, L Jagan Mohan Rao

¹Plantation Products, Spices and Flavor Technology Department, Central Food Technological Research Institute, Mysore, India.

Journal of Chromatographic Science

|May 17, 2007

Summary

A new semi-preparative high-performance liquid chromatography method effectively separates bioactive triterpenoids and their acetates from swallow root. This technique overcomes limitations of traditional chromatography for these valuable plant compounds.

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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

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Published on: October 4, 2019

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Last Updated: Jul 14, 2026

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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Area of Science:

Natural Product Chemistry
Phytochemistry
Analytical Chemistry

Background:

Decalepis hamiltonii (swallow root) is a rich source of bioactive triterpenoids.
Triterpenoids like alpha-amyrin, beta-amyrin, and lupeol, along with their acetates, possess potential health benefits.
Traditional chromatographic methods struggle to separate these complex mixtures from plant extracts.

Purpose of the Study:

To develop a convenient semi-preparative high-performance liquid chromatography (HPLC) method.
To achieve efficient separation of triterpenoids and their acetates from Decalepis hamiltonii.
To characterize the isolated compounds using advanced spectral techniques.

Main Methods:

Utilized semi-preparative reversed-phase HPLC under optimized conditions.
Employed hexane extraction and column chromatography for initial fractionation.
Characterized separated compounds using nuclear magnetic resonance (NMR) spectroscopy and analysis of physical/chemical properties.

Main Results:

Successfully separated mixtures of triterpenoids (alpha-amyrin, beta-amyrin, lupeol) and their acetates.
Demonstrated the superiority of HPLC over classical column chromatography and preparative thin-layer chromatography for this separation.
Confirmed compound identity and purity through comprehensive spectral analysis.

Conclusions:

The developed semi-preparative HPLC method is effective for isolating bioactive triterpenoids and acetates from swallow root.
This method provides a valuable tool for further research into the pharmacological properties of these compounds.
Decalepis hamiltonii represents a significant natural reservoir for these potentially therapeutic triterpenoids.