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Updated: Jul 14, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Published on: March 15, 2017
A high-performance liquid chromatography with UV-vis detection method for the determination of Brazilein in plant
Xiaoling Yan1, Wei Wang, Lujun Zhang
1Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Abstract:
A previously established mobile phase composition for a high-performance liquid chromatography (HPLC)-electrochemical detection (ED) method for the determination of Brazilein is further examined by HPLC-UV. Each component in the mobile phase is evaluated by the HPLC-UV system, and a convenient HPLC-UV method for the determination of Brazilein is developed. The mobile phase of this HPLC-UV method consists of sodium dihydrogen phosphate (NaH(2)PO(4)). The detection limit of Brazilein (signal-to-noise = 3) is 1 ng, and the calibration graph of Brazilein ranges from 1-120 ng per 20-microL injection. The validity of the proposed HPLC-UV method is demonstrated by the analysis of Brazilein in real plant samples. The differences between the HPLC-ED method and the proposed HPLC-UV method are given.
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