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Effects of budesonide and probiotics enemas on the systemic inflammatory response of rats with experimental colitis
Mardem Machado de Souza1, José Eduardo de Aguilar-Nascimento, Diana Borges Dock-Nascimento
1Department of Surgery, Federal University of Mato Grosso, 78045-390 Cuiabá, MT, Brazil.
Purpose:
The aim of this study was to investigate the effect of enemas containing probiotics and budesonide on the systemic inflammatory response in experimental colitis.
Methods:
Fifty male Wistar rats with experimental colitis induced by 10% acetic acid enema were randomized to five groups (10 rats each) according to the treatment: group 1--saline solution, group 2--budesonide (0.75 mg/kg/day), group 3--probiotics (1mg/day), group 4--probiotics plus budesonide, and group 5--control, with not-treated rats. The following variables were studied: body weight, serum levels of albumin, C-reactive protein and interleucine-6 (IL-6).
Results:
All animals lost weight between the beginning and the end of the experiment (280+ 16 mg versus 249+21 mg, p< 0.001). There was a significant decrease in the serum albumin between the normal pre-induction level (3.45 + 0.49 mg/dL) and the 1st day after colitis induction (1.61+051 mg/dL, p< 0.001) in all treated groups when compared to the control group. C- reactive protein increased after induction and diminished on the 7th day in all groups. In the control group there was an increase in the IL-6 after colitis induction. None of the treated groups significantly differed from IL-6 pre-colitis status (p>0.05). Only probiotic rats presented a significant decrease of IL-6 than controls (0,30+/-0,08 mg/dL vs. 0,19+/-0,03 mg/dL; p<0.01).
Conclusion:
Probiotics are effective to diminished inflammatory status mediated by IL-6 in experimental colitis.
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