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Related Experiment Videos

Biotinylated basic fibroblast growth factor is biologically active.

I Pieri1, D Barritault

  • 1Laboratoire de Biotechnologie des Cellules Eucaryotes, Université Paris XII, Créteil, France.

Analytical Biochemistry
|June 1, 1991
PubMed
Summary
This summary is machine-generated.

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Biotinylation of basic fibroblast growth factor (bFGF) created derivatives bio-bFGF.20 and bio-bFGF.200 that retained heparin affinity and biological activity. These modified bFGF forms effectively promoted cell growth, similar to native bFGF.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Basic fibroblast growth factor (bFGF) is crucial for cell growth and tissue repair.
  • Modifying bFGF can alter its properties for specific applications.
  • Biotinylation is a common technique for labeling proteins.

Purpose of the Study:

  • To create biotinylated basic fibroblast growth factor (bFGF) derivatives.
  • To evaluate the impact of biotinylation on bFGF's heparin affinity and receptor binding.
  • To assess the biological activity of biotinylated bFGF in cell proliferation.

Main Methods:

  • Basic fibroblast growth factor (bFGF) was biotinylated using biotin-N-hydroxysuccinimide ester at varying molar ratios.
  • Heparin affinity was measured for native and biotinylated bFGF.

Related Experiment Videos

  • Competition assays with 125I-bFGF were performed using bovine brain membranes to assess receptor binding.
  • Cell proliferation was evaluated on CCL39 cells using the modified bFGF derivatives.

    • Main Results:

    • Biotinylated bFGF derivatives (bio-bFGF.20 and bio-bFGF.200) retained heparin-binding affinity, unlike bio-bFGF.2000.
    • bio-bFGF.20 and bio-bFGF.200 demonstrated comparable binding to the bFGF receptor as native bFGF.
    • Both bio-bFGF.20 and bio-bFGF.200 effectively promoted CCL39 cell growth, similar to native bFGF.

    Conclusions:

    • Controlled biotinylation of bFGF yields derivatives with preserved biological function.
    • bio-bFGF.20 and bio-bFGF.200 are viable alternatives to native bFGF for applications requiring biotinylated forms.
    • The degree of biotinylation is critical for maintaining bFGF's functional properties.