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Updated: Jul 14, 2026

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
Oligonucleotide profiling for discriminating bacteria in bacterial communities
1College of Science, Zhejiang Sci-Tech University, Hangzhou, PR China. pinganhe@yahoo.com.cn
Abstract:
Based on the relative ratios of di- and tri-nucleotides in the DNA sequences, the profiles of 164 genome sequences from 152 representative microbial organisms were computed. By comparing the profiles of the genomes and their substrings with length 500 bps, the fluctuations of the relative abundances of di- and tri-nucleotides of these genomic sequences were analyzed. A new method to discriminate the origins of orphan DNA sequences was proposed, and the origins of 17 uncultured bacterium sequences from a bacterial community in the human gut were postulated and discussed.
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