Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Connexin-dependent transcellular transcriptomic networks in mouse brain.

Dumitru A Iacobas1, Sanda Iacobas, David C Spray

  • 1Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, New York, NY, USA. diacobas@aecom.yu.edu

Progress in Biophysics and Molecular Biology
|May 18, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo characterization of a patient CACNA1A variant reveals paradoxical synaptic effects.

bioRxiv : the preprint server for biology·2026
Same author

Mitochondrial Collapse Responsible for Chagasic and Post-Ischemic Heart Failure Is Reversed by Cell Therapy Under Different Transcriptomic Topologies.

Current issues in molecular biology·2025
Same author

Neurotransmission Sex Dichotomy in the Rat Hypothalamic Paraventricular Nucleus in Healthy and Infantile Spasm Model.

Current issues in molecular biology·2025
Same author

Editorial: Glial cells in homeostasis, neurodevelopment, and repair.

Frontiers in cellular neuroscience·2025
Same author

Mental disorders after myocardial infarction: potential mediator role for chemokines in heart-brain interaction?

Journal of geriatric cardiology : JGC·2024
Same author

Astrocytes sense glymphatic-level shear stress through the interaction of sphingosine-1-phosphate with Piezo1.

iScience·2024
Same journal

Mathematical Frameworks for Left Ventricular Assist Device Therapy: Ventricular Mechanics, Blood Rheology, Haemodynamics, Control, and Nonlinear Dynamics.

Progress in biophysics and molecular biology·2026
Same journal

Biological functions of BAF57, its role in disease pathogenesis, and treatment: From molecular mechanisms to clinical translation.

Progress in biophysics and molecular biology·2026
Same journal

Photonics-integrated and AI-enhanced medical sensing: From molecular diagnostics to real-time cell therapy monitoring.

Progress in biophysics and molecular biology·2026
Same journal

Uncovering the Biological Mechanisms of TREM2 with Molecular Simulations: A Comprehensive Review and Perspective.

Progress in biophysics and molecular biology·2026
Same journal

Advances in artificial joint testing driven by in situ mechanical characterization: From permeability of porous structures to dynamic wear monitoring.

Progress in biophysics and molecular biology·2026
Same journal

Proteostasis-driven redox adaptation in ferroptosis: the p62-Keap1-Nrf2 axis.

Progress in biophysics and molecular biology·2026
See all related articles

Gene expression networks in mouse brains are regulated by connexins (Cx). Deleting connexin genes reveals distinct neuronal and glial networks, impacting transcription factor regulation and downstream pathways.

Area of Science:

  • Neuroscience
  • Genomics
  • Molecular Biology

Background:

  • Microarray studies typically focus on gene expression magnitude.
  • Novel strategies can extract additional information like expression variability and coordination.
  • Connexins (Cx) are crucial for cell-cell communication via gap junctions.

Purpose of the Study:

  • To investigate transcriptomic organizational principles beyond simple expression levels.
  • To explore the role of specific connexins (Cx43, Cx32, Cx36) in regulating gene expression networks in the brain.
  • To identify connexin-dependent transcriptomic networks and their impact on transcription factor regulation.

Main Methods:

  • Analysis of gene expression variability and coordination in microarray data.
  • Comparison of gene expression patterns in wildtype and connexin-null (Gja1(-/-), Gjb1(-/-), Gja9(-/-)) mouse brains.

Related Experiment Videos

  • Identification of overlapping and distinct gene regulations across different connexin knockout models.

    • Main Results:

    • Gene expression levels, variability, and coordination are regulated in connexin43 null (Gja1(-/-)) mouse brains.
    • Expression regulation in Gja1(-/-) largely mirrored wildtype coordination patterns.
    • Significant overlap in gene regulation was observed between Gja1(-/-) and connexin32 null (Gjb1(-/-)) brains, distinct from connexin36 null (Gja9(-/-)) brains.
    • These findings suggest distinct connexin-dependent panglial and neuronal transcriptomic networks.

    Conclusions:

    • Connexin-dependent transcriptomic networks exist in the brain, differing between glial and neuronal cell types.
    • Rearrangement of these networks in knockout models explains downstream phenotypic changes.
    • Transcription factors act as network hubs, enabling cross-pathway regulation.