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Updated: Jul 14, 2026
08:12
High-throughput Screening for Protein-based Inheritance in S. cerevisiae
Published on: August 8, 2017
Ure2p function is enhanced by its prion domain in Saccharomyces cerevisiae
Frank Shewmaker1, Lori Mull, Toru Nakayashiki
1Laboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830, USA.
Genetics
|May 18, 2007
Summary
The Ure2 protein
Area of Science:
- Molecular biology
- Yeast genetics
- Prion biology
Background:
- Ure2 protein in Saccharomyces cerevisiae can form a prion, [URE3].
- The [URE3] prion impairs nitrogen regulation and yeast growth.
- The role of Ure2p's N-terminal prion domain was unclear, especially its evolutionary conservation.
Purpose of the Study:
- Investigate the in vivo function and stability of Ure2p.
- Clarify the significance of the N-terminal prion domain in Ure2p.
- Explore potential interactions of the prion domain within the nitrogen regulation system.
Main Methods:
- Genetic analysis of Ure2p variants in Saccharomyces cerevisiae.
- Assessment of Ure2p protein stability and function in vivo.
- Investigation of protein-protein interactions involving Ure2p.
Main Results:
- Truncation of Ure2p's prion domain compromises its in vivo function.
- The full-length prion domain is essential for Ure2p stability.
- Ure2p's prion domain may influence interactions with Gzf3p, a nitrogen regulation component.
Conclusions:
- The N-terminal prion domain of Ure2p is crucial for its stability and function.
- The prion domain plays a role beyond prion formation, potentially in protein interactions.
- Understanding Ure2p's prion domain sheds light on prion biology and protein regulation.
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