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Updated: Jul 14, 2026

Cell Capture Using a Microfluidic Device

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

Microfluidic device for cell capture and impedance measurement.

Ling-Sheng Jang¹, Min-How Wang

¹Department of Electrical Engineering and Center for Micro/Nano Science and Technology, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan. lsjang@ee.ncku.edu.tw

Biomedical Microdevices

|May 18, 2007

Summary

This study introduces a microfluidic device for single cell impedance measurement. The device successfully captures HeLa cells and characterizes their electrical properties using impedance spectroscopy.

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Area of Science:

Biomedical Engineering
Microfluidics
Cellular Biophysics

Background:

Single-cell analysis is crucial for understanding cellular heterogeneity.
Impedance spectroscopy offers a label-free method for probing cell electrical properties.

Purpose of the Study:

To develop and validate a microfluidic device for single HeLa cell capture and impedance measurement.
To investigate the electrical characteristics of a single HeLa cell using impedance spectroscopy.

Main Methods:

Fabrication of a microfluidic device with integrated electrodes and micro pillars.
Utilizing CFD-ACE+ software for flow simulation and cell capture probability analysis.
Performing impedance spectroscopy on captured single HeLa cells across a frequency range of 1-100 kHz.

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Published on: February 4, 2011

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Last Updated: Jul 14, 2026

Cell Capture Using a Microfluidic Device

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Main Results:

The microfluidic device demonstrated successful capture of single HeLa cells.
An equivalent circuit model accurately represented the device and cell impedance.
HeLa cell impedance magnitude decreased with frequency and was influenced by operating voltage.

Conclusions:

The developed microfluidic system is effective for single-cell impedance characterization.
HeLa cell impedance is frequency-dependent and sensitive to applied electric fields.
The study provides insights into the electrical behavior of cancer cells at the single-cell level.