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Related Experiment Videos

Automatic microfluidic platform for cell separation and nucleus collection.

Chien-Hsuan Tai1, Suz-Kai Hsiung, Chih-Yuan Chen

  • 1Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan.

Biomedical Microdevices
|May 18, 2007
PubMed
Summary
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This study presents an automated microfluidic biochip using dielectrophoresis (DEP) for cell separation and nucleus collection. The device achieves high throughput and accuracy, enabling nuclear protein extraction for research.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Automated cell separation and nucleus collection are crucial for biological research and diagnostics.
  • Existing methods often lack efficiency, throughput, or require manual intervention.

Purpose of the Study:

  • To develop and validate an integrated microfluidic biochip for automated cell separation and nucleus collection using dielectrophoresis (DEP).
  • To assess the device's performance in separating viable and non-viable cells and collecting nuclei.

Main Methods:

  • Integration of microfluidic components including micropumps, microvalves, and DEP electrodes on a micro-electro-mechanical-systems (MEMS) chip.
  • Utilizing dielectrophoresis (DEP) forces at low voltage (15 Vp-p) and specific frequency (16 MHz) for cell separation.

Related Experiment Videos

  • Employing a serpentine-shape (S-shape) pneumatic micropump for continuous sample transport and microvalves for automated sorting.

    • Main Results:

    • Successful separation of viable and non-viable human lung cancer cells (A549-luc-C8) with accuracies of 84% and 81%, respectively.
    • Demonstrated collection of cell nuclei after cell lysis using the same microfluidic platform.
    • Achieved a pumping rate of 39.8 µL/min and a cell separation throughput of 240 cells/min.

    Conclusions:

    • The developed automated microfluidic platform offers an efficient and accurate method for cell separation and nucleus collection.
    • The platform is suitable for extracting nuclear proteins from living cells for downstream applications like binding assays.