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Related Experiment Video

Updated: Jul 14, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

Osteosarcoma cell-calcium signaling through tissue factor-factor VIIa complex and factor Xa.

Valéry Daubie¹, Robert De Decker, Charles Nicaise

¹Laboratory of Histology, Neuroanatomy and Neuropathology, CP620, Université Libre de Bruxelles Route de Lennik 808, Bruxelles, Belgium. valery.daubie@ulb.ac.be

|May 19, 2007

Summary

Bone cells express protease-activated receptors. Tissue factor and factor Xa activate these receptors, influencing intracellular calcium levels and cell viability in osteoblasts.

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Published on: May 6, 2018

Area of Science:

Biochemistry
Cell Biology
Bone Physiology

Background:

Bone formation involves cells expressing protease-activated receptors (PARs).
Thrombin, a serine protease, affects bone cell survival and alkaline phosphatase activity.
The roles of upstream coagulation factors (tissue factor, factor VIIa, factor Xa) in bone cells are unknown.

Purpose of the Study:

To investigate the expression and function of tissue factor, factor VIIa, and factor Xa in osteoblast-like cells.
To determine if these factors activate PARs and influence intracellular calcium signaling.
To examine the impact of factor Xa and PAR activation on osteoblast function.

Main Methods:

Utilized the osteoblast-like cell line SaOS-2.
Assessed tissue factor expression in SaOS-2 cells.
Investigated the effects of tissue factor/factor VIIa complexes and factor Xa on intracellular calcium (Ca2+) levels.
Examined the impact of a PAR2-activating peptide (SLIGRL) and PAR-1 activation on cell viability.

Main Results:

Tissue factor is expressed in SaOS-2 cells.
A tissue factor/factor VIIa complex induced a transient intracellular Ca2+ increase via PAR2.
Factor Xa elicited a sustained intracellular Ca2+ response in SaOS-2 cells.
PAR2 activation by SLIGRL and PAR-1 activation affected cell viability.

Conclusions:

Osteoblast-like cells express tissue factor, a component of the coagulation cascade.
Tissue factor and factor Xa can activate PARs in bone cells, modulating intracellular calcium signaling.
These findings suggest a novel role for coagulation factors in bone cell function and potentially bone remodeling.