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A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
Published on: December 2, 2009
RNA maps reveal new RNA classes and a possible function for pervasive transcription
Philipp Kapranov1, Jill Cheng, Sujit Dike
1Affymetrix Laboratory, Affymetrix, Inc., 3420 Central Expressway, Santa Clara, CA, 95051, USA.
Science (New York, N.Y.)
|May 19, 2007
Summary
This study reveals that unannotated RNAs play a crucial role in producing short RNAs. These findings highlight a complex, interleaved organization within the human transcriptome.
Area of Science:
- Genomics
- Molecular Biology
- Transcriptomics
Background:
- Eukaryotic genomes produce substantial amounts of unannotated RNA with limited protein-coding capacity.
- The function and origin of many RNA molecules remain poorly understood.
Purpose of the Study:
- To investigate the genomic origins and associations of specific human RNAs.
- To explore the processing of these RNAs into shorter forms and their subcellular localization.
Main Methods:
- Genome-wide analysis of polyadenylated RNAs (>200 nucleotides) and whole-cell RNAs (<200 nucleotides).
- Assignment of subcellular locations for RNA nucleotides.
- Investigation of RNA processing pathways.
Main Results:
- Identified novel roles for unannotated RNAs as precursors for short RNAs.
- Characterized three potentially functional RNA classes.
- Found two RNA classes to be syntenically conserved and linked to protein-coding gene expression.
Conclusions:
- Unannotated RNAs are significant in the biogenesis of short RNAs.
- The human transcriptome exhibits a highly interleaved organization.
- Discovered potentially functional and conserved RNA classes.
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