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Published on: December 11, 2020
'In vitro' protective effect of a hydrophilic vitamin E analogue on the decrease in levels of elongation factor 2 in
Sandro Argüelles1, Alberto Machado, Antonio Ayala
1Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, España.
Background:
Protein synthesis is inhibited by oxidative stress. Among the possible causes of this inhibition are the modifications of elongation factor 2 (eEF-2), the protein that catalyzes the translocation of the ribosome through mRNA. eEF-2 is extremely sensitive to oxidative stress caused mainly by lipid peroxidant compounds such as cumene hydroperoxide (CH).
Objective:
The purpose of this study was to determine whether the antioxidant Trolox prevents the effect of CH on the levels of hepatic eEF-2.
Methods:
The effect was determined in liver homogenates treated with both compounds. Lipid peroxides and carbonyl content were also measured.
Results:
The results show that Trolox at certain doses prevents the decrease in the level of eEF-2 caused by CH.
Conclusion:
Under oxidative stress circumstances, vitamin E can prevent the effect of oxidations on relevant biological processes such as protein synthesis.
