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Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
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Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

The post-genomic era: workshop on chromatin immunoprecipitation-related techniques

G Blandino1, M Fanciulli, M Levrero

  • 1Rome Oncogenomic Center, Istituto Regina Elena, Via delle Messi D'Oro, Rome, Italy. blandino@ifo.it

Cell Death and Differentiation
|May 19, 2007
PubMed
Abstract

No abstract available in PubMed .

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Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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