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Surface-plasmon-enhanced fluorescence spectroscopy for DNA detection using fluorescently labeled PNA as "DNA

Li-Qiang Chu1, Renate Förch, Wolfgang Knoll

  • 1Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128, Mainz, Germany.

Angewandte Chemie (International Ed. in English)
|May 19, 2007
PubMed
Summary

No abstract available in PubMed .

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...