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[Circulated extraction of flavonoids from Glechoma longituba by ultrasonic wave]
1Jinzhou Medical College, Jinzhou 121001, China.
Objective:
To investigate the optimum condition of extraction for the flavonoids in Glechoma longituba by ultrasonic wave.
Method:
Using orthogonal test, the effects of ultrasonic power, ultrasonic time, extraction temperature and solvent concentration were considered, the comprehensive evaluation was guided by the content of the flavonoids determined by ultraviolet spectrophotometer.
Result:
The optimum condition was as follow: ultrasonic power; 800 W, ultrasonic time 90 min, extraction temperature 40 degrees C, solvent concentration 65%. The flavonoids concent is 5.228%.
Conclusion:
Using ethanol as solvent, circulated extraction of the flavonoids from G. longituba with ultrasonic wave is feasible with the optimum conditions in low temperature, short time and high production yield.