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Updated: Jul 14, 2026
Generation and Manipulation of Rat Intestinal Organoids
Published on: June 23, 2023
[Effect of emodin on proliferation and differentiation of rat preadipocytes]
Yong-Qing Yang1, Gong-She Yang
1Laboratory of Fat Deposition and Muscle Development, North West Sci-tech University of Agriculture and Foristry, Yangling 712100, China.
Objective:
To study the effect of emodin (EMO) on the proliferation and differentiation of rat preadipocytes.
Method:
Separating and culturing rat preadipocytes, grouping the wells that preadipocytes were growing according to certain concentration such as 0, 5, 10, 20, 40, 80, 160 micromol x L(-1) randomly, MTT spectrophotometry and flow cytometry (FCM) were adopted to determine the effect of EMO on proliferation of rat preadipocytes. The accumulation of TG (triglyceride) in adipocytes was assayed by oil red O staining, and the morphological changes of the adipocytes were determined by morphology observation.
Result:
EMO in the range of 20-160 micromol x L(-1) could inhibit the proliferation and differentiation of preadipocytes in a dose and time dependent manner, and induce apoptosis of preadipocytes in a certain degree.
Conclusion:
EMO should have a potential to serve as a fat-reducing drug.
