Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Effects of L-carnitine on RBC membrane composition and function in hyperinsulinemic rats.

P Rajasekar1, K Balasaraswathi, C V Anuradha

  • 1Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Tamil Nadu, India.

The Italian Journal of Biochemistry
|May 22, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of the hysteretic thermal properties of W-doped and undoped nanocrystalline powders of VO<sub>2</sub>.

Scientific reports·2019
Same author

Indirubin-3'-monoxime prevents aberrant activation of GSK-3β/NF-κB and alleviates high fat-high fructose induced Aβ-aggregation, gliosis and apoptosis in mice brain.

International immunopharmacology·2019
Same author

Biocompatibility and antibacterial activity of the Adathoda vasica Linn extract mediated silver nanoparticles.

Microbial pathogenesis·2016
Same author

An intrauterine catch-up growth regimen increases food intake and post-natal growth in rats.

Journal of animal physiology and animal nutrition·2014
Same author

Nitric oxide mediates the insulin sensitizing effects of β-sitosterol in high fat diet-fed rats.

Nitric oxide : biology and chemistry·2013
Same author

Hepatoprotective action of celery (Apium graveolens) leaves in acetaminophen-fed freshwater fish (Pangasius sutchi).

Fish physiology and biochemistry·2013
Same journal

Alpha-bisabolol: unexpected plant-derived weapon in the struggle against tumour survival?

The Italian journal of biochemistry·2009
Same journal

Expression of succinate dehydrogenase flavoprotein subunit in saccharomyces cerevisiae studied by lacZ reporter strategy. Effect of FLX1 deletion.

The Italian journal of biochemistry·2009
Same journal

The smaller isoform of the mitochondrial transcription factor A has a role in the mitochondrial transcription.

The Italian journal of biochemistry·2009
Same journal

Functional proteomics: protein-protein interactions in vivo.

The Italian journal of biochemistry·2009
Same journal

Synergistic effect of Ca2+ and peroxidized cardiolipin in the induction of permeability transition and cytochrome c release in rat heart mitochondria.

The Italian journal of biochemistry·2009
Same journal

Neurosteroid-growth factor cross-talk induces up and down regulation of GFAP and vimentin expression in serum free astrocyte cultures.

The Italian journal of biochemistry·2009
See all related articles

L-carnitine (CA) protects red blood cells (RBCs) from damage caused by high fructose diets. CA helps maintain RBC membrane integrity and reduces oxidative stress in fructose-fed rats.

Area of Science:

  • Biochemistry
  • Physiology
  • Nutritional Science

Background:

  • High fructose diets induce hyperinsulinemia and hyperglycemia.
  • These conditions cause significant alterations in red blood cell (RBC) membrane composition and function.
  • Oxidative stress and increased susceptibility to lipid oxidation are observed in RBCs of fructose-fed rats.

Purpose of the Study:

  • To investigate the effects of L-carnitine (CA) on erythrocyte (RBC) susceptibility to peroxide-induced lipid oxidation.
  • To assess CA's impact on RBC membrane composition, ATPases activity, and oxidative stress markers.
  • To evaluate CA's protective role in a rat model of diet-induced type 2 diabetes.

Main Methods:

  • Induction of experimental hyperinsulinemia and hyperglycemia in rats via a high fructose diet (60 g/100 g) for 6 weeks.

Related Experiment Videos

  • Analysis of RBC membrane composition, including protein, cholesterol, phospholipids, free fatty acids, and carbohydrates.
  • Measurement of membrane-bound ATPases activity (total, Na+/K+, Mg2+, Ca2+), thiobarbituric acid reactive substances (TBARS), and lipid hydroperoxides (LHP).
  • Assessment of RBC susceptibility to peroxide-induced oxidative stress and vitamin E levels.
  • Treatment of fructose-fed rats with L-carnitine (300 mg/kg b.w/day, i.p.).

    • Main Results:

    • Fructose feeding led to decreased protein content and increased cholesterol, phospholipids, and free fatty acids in RBC membranes.
    • Significant alterations in RBC membrane carbohydrate composition were observed.
    • Reduced activity of membrane-bound ATPases and elevated levels of TBARS and LHP indicated increased oxidative stress.
    • RBCs from fructose-fed rats showed increased susceptibility to peroxide-induced oxidative stress, correlating with lower vitamin E levels.
    • L-carnitine treatment prevented fructose-induced alterations in membrane composition and enzyme activities.
    • CA alleviated lipid peroxidation caused by fructose loading.

    Conclusions:

    • High dietary fructose intake is detrimental to RBC membrane integrity.
    • L-carnitine demonstrates membrane-stabilizing effects in a diet-induced model of type 2 diabetes.
    • CA may offer a protective strategy against fructose-induced oxidative stress and RBC damage.