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Updated: Jul 14, 2026
Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
Published on: September 1, 2018
Dienogest inhibits BrdU uptake with G0/G1 arrest in cultured endometriotic stromal cells
Li Fu1, Yutaka Osuga, Chieko Morimoto
1Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Objective:
To investigate the effect of dienogest on the proliferation of endometriotic stromal cells.
Design:
Comparative and laboratory study.
Setting:
University of Tokyo Hospital.
Patient(S):
Endometriotic stromal cells were isolated and cultured from ovarian endometriomas of patients undergoing surgery.
Intervention(S):
Dienogest was added to the cultured endometriotic stromal cells.
Main Outcome Measure(S):
5-Bromo-2'-deoxyuridine (BrdU) incorporation into DNA of the endometriotic stromal cells was measured by ELISA. Cell cycle analysis of the cultured endometriotic stromal cells was performed by flow cytometry.
Result(S):
Dienogest at concentration of 10(-7) M and 10(-6) M significantly inhibited BrdU incorporation into DNA at 24 and 48 hours. Dienogest significantly increased the cells in G0/G1 phase and reduced the cells in S phase and G2/M phase in 24 and 48 hours.
Conclusion(S):
The present study indicates that dienogest can inhibit the proliferation of the endometriotic stromal cells with G0/G1 arrest, suggesting a possible direct effect of dienogest in the treatment of endometriosis.
Insights
Dienogest effectively inhibits endometriotic stromal cell proliferation by halting cell cycle progression. This finding suggests dienogest may directly treat endometriosis by impacting these specific cells.
Area of Science:
- Reproductive biology
- Cell biology
- Pharmacology
Background:
- Endometriosis is a condition characterized by the growth of endometrial-like tissue outside the uterus.
- Endometriotic stromal cells play a crucial role in the pathogenesis and progression of endometriosis.
- Understanding the cellular mechanisms underlying endometriosis is vital for developing effective treatments.
Purpose of the Study:
- To investigate the direct effect of dienogest on the proliferation of human endometriotic stromal cells.
- To determine if dienogest influences the cell cycle distribution of endometriotic stromal cells.
Main Methods:
- Endometriotic stromal cells were isolated from ovarian endometriomas and cultured in vitro.
- Cells were treated with varying concentrations of dienogest.
- Cell proliferation was assessed by measuring 5-Bromo-2'-deoxyuridine (BrdU) incorporation.
- Cell cycle analysis was performed using flow cytometry.
Main Results:
- Dienogest significantly inhibited endometriotic stromal cell proliferation at concentrations of 10(-7) M and 10(-6) M.
- Inhibition of proliferation was observed at 24 and 48 hours post-treatment.
- Dienogest treatment led to an accumulation of cells in the G0/G1 phase.
- A reduction in the proportion of cells in the S and G2/M phases was noted.
Conclusions:
- Dienogest demonstrates a direct inhibitory effect on endometriotic stromal cell proliferation.
- The mechanism involves the arrest of the cell cycle at the G0/G1 phase.
- These findings support the potential of dienogest as a therapeutic agent for endometriosis.
