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Evidence of mononuclear cell activation by hemodialysis

D Donati1, D Degiannis, N Combates

  • 1Department of Pathology, UMDNJ-Robert Wood Johnson Medical School, Piscataway.

ASAIO Transactions
|July 11, 1991
PubMed

Insights

Hemodialysis (HD) activates mononuclear cells (MC) by inducing interleukin-2 receptor (IL-2R) mRNA, but does not alter IL-2R expression or IL-2 responsiveness. HD also stimulates interleukin-1 (IL-1) synthesis.

Area of Science:

  • Immunology
  • Nephrology
  • Biochemistry

Background:

  • Mononuclear cells (MC) play a crucial role in immune responses.
  • Hemodialysis (HD) is a vital treatment for kidney failure.
  • Understanding HD's impact on immune cells is essential for patient care.

Purpose of the Study:

  • To investigate the acute effects of hemodialysis on mononuclear cell activation and function.
  • To compare the effects of cuprophan (CU) and polysulfone (PS) membranes on MC during in vitro HD.
  • To assess the impact of HD on interleukin-2 receptor (IL-2R) expression, soluble IL-2R (sIL-2R) release, and cellular responsiveness.

Main Methods:

  • In vitro hemodialysis model using cuprophan and polysulfone membranes.
  • Analysis of interleukin-2 receptor (IL-2R) mRNA transcription.
  • Flow cytometry to assess IL-2R expression on the cell membrane.
  • Measurement of soluble IL-2R (sIL-2R) levels in the supernatant.
  • Assays for interleukin-2 (IL-2) and interleukin-1 (IL-1) production.
  • Assessment of mononuclear cell proliferative response to phytohemagglutinin.

Main Results:

  • Both cuprophan and polysulfone induced interleukin-2 receptor (IL-2R) mRNA transcription during hemodialysis (HD).
  • HD did not alter IL-2R membrane expression, soluble IL-2R (sIL-2R) release, or IL-2 responsiveness.
  • Polysulfone slightly reduced proliferative response to phytohemagglutinin and sIL-2R release.
  • Hemodialysis induced interleukin-1 (IL-1) synthesis but not IL-2 production.

Conclusions:

  • Hemodialysis acutely affects mononuclear cells by inducing IL-2R mRNA and IL-1 synthesis.
  • The studied HD membranes do not significantly impair key aspects of mononuclear cell function like IL-2R expression or IL-2 responsiveness.
  • Further research may explore the clinical implications of HD-induced IL-1 synthesis on immune status.

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