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Updated: Jul 14, 2026
Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
Published on: August 27, 2016
Fluorescence activated cell sorting (FACS) using RNAlater to minimize RNA degradation and perturbation of mRNA
Kevin P Nishimoto1, Daniel Newkirk, Stephen Hou
1Molecular Biology and Biochemistry, School of Biological Sciences, University of California, Irvine, Irvine, CA 92697, USA.
Abstract:
Host microbe interactions frequently involve specific cellular tropism. Accurate characterization of cells involved in these initial interactions is complicated by the response to the microbe. We describe a method utilizing RNAlater for Fluorescence Activated Cell Sorting of these critical cells that minimizes the downstream perturbation in the gene expression profile.
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