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Updated: Jul 14, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Limitations of transferability of absolute cut-points in non-standardised assays

Adie Viljoen, Patrick J Twomey

    Journal of Clinical Pathology
    |May 22, 2007
    PubMed
    Abstract

    No abstract available in PubMed .

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    The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
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