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Noncontact optical imaging in mice with full angular coverage and automatic surface extraction.

Heiko Meyer1, Anikitos Garofalakis, Giannis Zacharakis

  • 1Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas, Heraklion Crete, Greece. heimeyer@iesl.forth.gr

Applied Optics
|May 22, 2007
PubMed
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This study introduces a new noncontact 3D imaging system for fluorescence molecular tomography (FMT) and diffuse optical tomography (DOT). The system enables quantitative in vivo imaging for small animal research.

Area of Science:

  • Biomedical Imaging
  • Optical Physics
  • Medical Physics

Background:

  • Diffuse optical tomography (DOT) and fluorescence molecular tomography (FMT) provide quantitative 3D maps of in vivo chromophore/fluorophore concentrations.
  • Advances in DOT/FMT rely on large datasets from CCD detectors and noncontact multi-projection methods.
  • Existing methods require specialized setups for arbitrary shapes and small animal imaging.

Purpose of the Study:

  • To develop and evaluate a novel noncontact 3D imaging system for DOT and FMT.
  • To enable arbitrary shape imaging in fluorescence or absorption modes for small animal studies.
  • To improve the reconstruction of in vivo fluorescence data and concentrations in scattering tissues.

Main Methods:

  • Developed a noncontact imaging setup using CCD cameras for both detection and surface geometry acquisition.

Related Experiment Videos

  • Implemented thresholded shadowgrammetry to reconstruct the 3D surface mesh.
  • Applied free-space propagation equations to map in vivo fluorescence data onto the tissue surface.
  • Reconstructed fluorescence concentrations within scattering phantom samples.

    • Main Results:

    • Successfully recovered 3D surfaces from phantom data and live mice.
    • Demonstrated the system's capability for in vivo fluorescence mapping and internal concentration reconstruction.
    • Validated the noncontact approach for both sources and detectors.

    Conclusions:

    • The developed imaging system offers a versatile platform for 3D DOT and FMT in small animal research.
    • This noncontact approach enhances data acquisition and surface geometry registration.
    • The system has significant potential to advance biomedical research through improved in vivo optical imaging.