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In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2
Published on: June 13, 2020
Using photoactivatable fluorescent protein Dendra2 to track protein movement
Dmitriy M Chudakov1, Sergey Lukyanov, Konstantin A Lukyanov
1Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Biotechniques
|May 23, 2007
Summary
Photoactivatable fluorescent proteins like Dendra2 can change color with light, aiding protein mobility studies. This work offers guidance on using Dendra2 for tracking proteins in live cells.
Area of Science:
- Biochemistry
- Cell Biology
- Biophysics
Background:
- Photoactivatable fluorescent proteins exhibit light-induced changes in fluorescence.
- These proteins are valuable tools for studying protein dynamics and cellular processes.
- Dendra2 is a monomeric green-to-red photoconvertible fluorescent protein.
Purpose of the Study:
- To provide detailed recommendations for using Dendra2.
- To facilitate protein tracking in living cultured cells.
Main Methods:
- Application of the monomeric green-to-red photoconvertible fluorescent protein Dendra2.
- Protein tracking in living cultured cells.
Main Results:
- Dendra2 enables effective protein tracking in live cells.
- Recommendations for optimal application are provided.
Conclusions:
- Dendra2 is a powerful tool for protein mobility studies.
- Detailed guidelines enhance its utility in live-cell imaging.
