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Phenotypic and genotypic characterization of Paenibacillus larvae isolates
K Antúnez1, C Piccini, S Castro-Sowinski
1Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avda. Italia 3318, C.P. 11600 Montevideo, Uruguay.
Veterinary Microbiology
|May 23, 2007
Summary
Two Paenibacillus larvae genotypes were identified in Uruguay, suggesting pathogen movement between Argentina and Uruguay. This study aids in understanding American Foulbrood (AFB) epidemiology in the region.
Area of Science:
- Microbiology
- Apiculture
- Epidemiology
Background:
- Paenibacillus larvae causes American Foulbrood (AFB), a significant honeybee disease.
- Understanding P. larvae epidemiology is crucial for bee health management.
Purpose of the Study:
- To develop a strategy for subtyping and epidemiological analysis of P. larvae.
- To investigate the genetic diversity and antibiotic susceptibility of P. larvae isolates in Uruguay and Argentina.
Main Methods:
- Phenotypic characterization
- Antibiotic susceptibility testing
- Repetitive element palindromic PCR (rep-PCR)
- Ribotyping
- Denaturing Gradient Gel Electrophoresis (DGGE)
Main Results:
- Two P. larvae genotypes, ERIC I-BOX A and ERIC I-BOX C, were identified in Uruguay.
- ERIC I-BOX C was previously exclusive to Argentina, indicating cross-border pathogen movement.
- rep-PCR, ribotyping, and DGGE did not significantly improve intraspecific discrimination.
- All isolates were oxytetracycline (OTC)-sensitive, but 22% of ERIC I-BOX A isolates were sulfisoxazole-resistant.
Conclusions:
- The findings suggest pathogen exchange between Argentina and Uruguay, likely via the Uruguay River.
- This study provides insights into the epidemiology of AFB in the region.
- Antibiotic resistance patterns may vary between P. larvae genotypes.
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