Related Experiment Videos
Molecular characterisation of kappa- and lambda-carrageenan by gel permeation chromatography, light scattering,
D Slootmaekers1, J A van Dijk, F A Varkevisser
1Department of Physical and Macromolecular Chemistry, Gorlaeus Laboratories, University of Leiden, Netherlands.
Abstract:
Gel permeation chromatography, in conjunction with a double detection system involving a low angle laser light scattering apparatus (LALLS) and a refractive index monitoring device (RI), has been used to obtain both the molecular weight and the molecular weight distribution of sodium salts of kappa-carrageenan and lambda-carrageenan in saline solutions. The results, Mw and Mn, are in excellent agreement with independent determinations of molar mass based on static light scattering experiments, sedimentation-diffusion analysis and osmometry. The relevance of the data is discussed with respect to current problems in carrageenan research.