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Related Experiment Videos

Silver-protein (core-shell) nanoparticle production using spent mushroom substrate.

Nadanathangam Vigneshwaran1, Arati A Kathe, Perianambi V Varadarajan

  • 1Nanotechnology Research Group, Central Institute for Research on Cotton Technology, Adenwala Road, Matunga, Mumbai 400 019, India. nvw75@yahoo.com

Langmuir : the ACS Journal of Surfaces and Colloids
|May 24, 2007
PubMed
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Researchers developed a green synthesis for silver-protein nanoparticles using spent mushroom substrate (SMS). This eco-friendly method yields stable nanoparticles with antibacterial properties against common pathogens.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Green Chemistry

Background:

  • Silver nanoparticles (AgNPs) possess significant antimicrobial properties but their synthesis often involves toxic chemicals.
  • Developing sustainable and cost-effective methods for AgNP synthesis is crucial for broader applications.
  • Spent mushroom substrate (SMS) is an abundant agricultural waste with potential for biomanufacturing.

Purpose of the Study:

  • To demonstrate a simple and eco-friendly synthesis of silver-protein (core-shell) nanoparticles using SMS.
  • To characterize the synthesized silver nanoparticles and their protein shell.
  • To evaluate the antibacterial efficacy and stability of the resulting nanoparticles.

Main Methods:

  • Incubation of silver nitrate with SMS to facilitate reduction and stabilization.

Related Experiment Videos

  • UV-Vis spectroscopy to confirm nanoparticle formation via surface plasmon resonance (SPR).
  • X-ray diffraction (XRD) for crystalline structure analysis, Transmission Electron Microscopy (TEM) for size and morphology, FTIR for protein shell confirmation.

    • Main Results:

    • Yellow coloration observed from 24h, with SPR peak at 436 nm, confirming AgNP formation.
    • TEM analysis revealed uniform nanoparticles (30.5 ± 4.0 nm) with a face-centered cubic (fcc) structure.
    • FTIR and fluorescence spectroscopy confirmed the presence of a stabilizing protein shell, and nanoparticles remained stable for over 6 months.
    • Significant antibacterial activity demonstrated against Staphylococcus aureus and Klebsiella pneumoniae.

    Conclusions:

    • Spent mushroom substrate (SMS) provides an effective and sustainable platform for synthesizing stable silver-protein nanoparticles.
    • The synthesized nanoparticles exhibit potent antibacterial activity, suggesting potential in biomedical and antimicrobial applications.
    • This biomanufacturing approach offers an ecofriendly alternative to conventional methods for nanoparticle production.