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Related Experiment Video

Updated: Jul 14, 2026

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging
10:03

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Published on: August 1, 2017

Imaging human bone marrow stem cell morphogenesis in polyglycolic acid scaffold by multiphoton microscopy.

Hsuan-Shu Lee1, Shu-Wen Teng, Hsiao-Ching Chen

  • 1Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University, College of Medicine, Taipei, Taiwan, ROC.

Tissue Engineering
|May 24, 2007
PubMed
Summary

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This study used multiphoton microscopy to track tissue engineering. Researchers visualized polyglycolic acid scaffolds, stem cells, and extracellular matrix formation noninvasively.

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Microscopy

Background:

  • Noninvasive imaging is crucial for assessing tissue engineering constructs.
  • Understanding physiological changes aids in designing better therapeutic products.

Purpose of the Study:

  • To image physiological changes in engineered human mesenchymal stem cell constructs.
  • To monitor extracellular matrix formation during chondrogenesis noninvasively.

Main Methods:

  • Combined multiphoton autofluorescence and second harmonic generation (SHG) microscopy.
  • Image human mesenchymal stem cells in polyglycolic acid scaffolds induced by transforming growth factor-beta3.

Main Results:

  • Multiphoton autofluorescence visualized the scaffold and cells.

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Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
09:34

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Published on: September 7, 2017

Related Experiment Videos

Last Updated: Jul 14, 2026

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging
10:03

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Published on: August 1, 2017

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
09:34

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Published on: September 7, 2017

  • SHG microscopy tracked extracellular matrix formation.
  • Extracellular matrix initially aligned with the scaffold, which reorganized over time.

    • Conclusions:

    • This combined microscopy approach allows noninvasive monitoring of tissue engineering processes.
    • The technique can track scaffold reorganization driven by chondrogenesis.
    • Potential for real-time monitoring of physiological changes in tissue engineering.