Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 14, 2026

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

Published on: October 17, 2016

Development of controlled matrix heterogeneity on a triphasic scaffold for orthopedic interface tissue engineering.

Jeffrey P Spalazzi¹, Stephen B Doty, Kristen L Moffat

¹Biomaterials and Interface Tissue Engineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, New York, USA.

Tissue Engineering

|May 24, 2007

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Return to Play, Performance, and Career Longevity After Shoulder Labral Repair in the National Football League: A Matched Cohort Analysis.

Orthopaedic journal of sports medicine·2026

Same author

Injury and Economic Burden of Shoulder and Elbow Injuries in Major League Baseball Pitchers: A Retrospective 11-Year Analysis (2015-2025).

Orthopaedic journal of sports medicine·2026

Same author

Advocacy for Medical Students Applying to Orthopaedic Surgery: A Practical Guide for Attending Physicians.

The Journal of the American Academy of Orthopaedic Surgeons·2026

Same author

Outcomes of reverse total shoulder arthroplasty in patients ≤65 years old.

Journal of shoulder and elbow arthroplasty·2026

Same author

Medical Retirement From Sport After Concussion: A Survey of Perspectives and Experiences of Collegiate Physicians and Athletic Trainers.

Clinical journal of sport medicine : official journal of the Canadian Academy of Sport Medicine·2026

Same author

Return to Play and Performance After Shoulder Labral Repair in Major League Baseball Players.

Orthopaedic journal of sports medicine·2026

Developing a triphasic scaffold promotes functional integration of soft tissue grafts with bone. This tissue engineering approach aims to regenerate the native interface, improving biological fixation for procedures like anterior cruciate ligament reconstruction.

Area of Science:

Biomaterials Science
Orthopedic Surgery
Tissue Engineering

Background:

Biological fixation of soft tissue grafts to bone is a major clinical challenge, particularly for anterior cruciate ligament (ACL) reconstruction.
Current graft integration methods result in non-physiologic fibrovascular tissue, creating a weak interface and limiting clinical success.
Re-establishing the native multi-tissue interface (ligament, fibrocartilage, bone) is crucial for successful biological graft fixation.

Purpose of the Study:

To design and evaluate a triphasic scaffold system that mimics the native ACL-to-bone interface.
To investigate osteoblast-fibroblast interactions within a three-dimensional co-culture system on the triphasic scaffold.
To assess the scaffold's ability to support multi-tissue regeneration and guide the formation of a functional fibrocartilage interface.

More Related Videos

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Published on: August 7, 2016

Related Experiment Videos

Last Updated: Jul 14, 2026

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

Published on: October 17, 2016

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Published on: August 7, 2016

Main Methods:

Design of a novel triphasic scaffold system replicating the native ligament-bone interface structure.
In vitro evaluation of osteoblast and fibroblast interactions using a three-dimensional co-culture model on the scaffold.
Assessment of cell proliferation, migration, phenotypic matrix production, and extracellular matrix deposition over time.

Main Results:

The triphasic scaffold successfully supported cell proliferation, migration, and phenotypic matrix production.
Distinct cellular regions and phase-specific extracellular matrix deposition were maintained over time.
The scaffold demonstrated the feasibility of regenerating multiple tissue types on a single construct.

Conclusions:

The triphasic scaffold system shows potential for guiding the regeneration of an anatomically oriented and mechanically functional fibrocartilage interface.
Interface tissue engineering using this scaffold could enable robust biological fixation of soft tissue grafts to bone.
This approach offers a promising strategy to overcome current limitations in orthopedic soft tissue graft integration.