Related Experiment Video
Updated: Jul 14, 2026
11:22
Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots
Published on: May 21, 2013
Injectable tissue-engineered cartilage using a fibrin sealant
Jinsoon Chang1, Jk J Rasamny, Stephen S Park
1Department of Otolaryngology, Inje University, Seoul Paik Hospital, Seoul, South Korea.
Archives of Facial Plastic Surgery
|May 24, 2007
Summary
Injectable cartilage tissue engineering is possible using fibrin sealant. Higher fibrin concentrations improved neocartilage formation, showing potential for cartilage regeneration without adverse host reactions.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Tissue Engineering
Background:
- Injectable scaffolds are crucial for minimally invasive tissue regeneration.
- Fibrin sealants offer biocompatibility and tunable properties for cell delivery.
Purpose of the Study:
- To evaluate a commercial fibrin sealant as a vehicle for injectable cartilage tissue engineering.
- To determine the optimal fibrin glue concentration for neocartilage formation.
Main Methods:
- Autogenous rabbit chondrocytes were suspended in fibrin glue at varying concentrations.
- The cell-fibrin mixture was injected into rabbit nasal dorsa.
- Constructs were harvested at 8 weeks and analyzed histologically.
Main Results:
- Successful neocartilage formation was achieved in 5 of 15 rabbits.
- Higher fibrin glue concentrations correlated with successful cartilage creation.
- Histological analysis revealed neocartilage with native cartilage characteristics, including elastin, and no foreign body reaction.
Conclusions:
- Percutaneous injection of chondrocytes in fibrin glue is a viable method for tissue-engineered cartilage.
- Optimizing fibrinogen, thrombin, and chondrocyte concentrations is key for consistent cartilage regeneration.
