Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium-binding proteins in Aplysia neurons.

A Hermann1, T L Pauls, C W Heizmann

  • 1Department of Physiology, University of Salzburg, Austria.

Cellular and Molecular Neurobiology
|August 1, 1991
PubMed
Summary

Researchers identified novel calcium-binding proteins in Aplysia neurons. These proteins, with distinct molecular weights, may influence nerve cell electrical activity, offering insights into neuronal function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

H<sub>2</sub>S prevents the disruption of the blood-brain barrier in rats with prenatal hyperhomocysteinemia.

Journal of pharmacological sciences·2024
Same author

Open- and Closed-Loop Recycling: Highly Active Zinc Bisguanidine Polymerization Catalyst for the Depolymerization of Polyesters.

ChemSusChem·2024
Same author

Prevalence of suicidal ideation in German psychotherapy outpatients: A large multicenter assessment.

Journal of affective disorders·2024
Same author

Treatment of Pneumonitis: From the <i>Allgemeine Wiener Med. Zeitung</i>.

Medical examiner (Chicago, Ill.)·2023
Same author

Treatment of Pneumonitis: From the <i>Allgemeine Wiener Medical Zeitung</i>.

Medical examiner (Chicago, Ill.)·2023
Same author

Treatment of Pneumonitis: From the <i>Allgemeine Wiener Med. Zeitung</i>.

Medical examiner (Chicago, Ill.)·2023

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Calcium-binding proteins play crucial roles in cellular processes.
  • Understanding neuronal calcium-binding proteins is key to deciphering nerve cell function.
  • The marine mollusk Aplysia californica serves as a model organism for studying neuronal mechanisms.

Purpose of the Study:

  • To investigate calcium-binding proteins in neuronal ganglia and identified neurons of Aplysia californica.
  • To characterize the molecular properties and distribution of these proteins.
  • To explore the potential relationship between calcium-binding proteins and neuronal electrical activity.

Main Methods:

  • Transblot/45Ca overlays to identify Ca-binding proteins.
  • Western blot analysis and 2D-PAGE for protein separation and characterization.
  • Immunocross-reactivity assays to determine protein homology.

Main Results:

  • Two major Ca-binding proteins identified at Mr 45,000 and Mr 23,000.
  • The Mr 45,000 protein separated into Mr 40,000 and Mr 43,000 components, with the Mr 40,000 protein showing novel antigenic properties.
  • The Mr 23,000 protein exhibited similarity to sarcoplasmic calcium-binding proteins (SCP).
  • Distinct protein patterns, particularly in low molecular weight ranges, were observed in neurons with different electrical activities, with silent cells showing a protein similar to crayfish SCP.

Conclusions:

  • Aplysia neurons possess diverse calcium-binding proteins.
  • These proteins are characteristic of specific neuronal types and may modulate electrical behavior.
  • The findings contribute to understanding the molecular basis of neuronal excitability.

Related Experiment Videos