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Related Experiment Videos

A bioactive polyacetylene compound isolated from Centella asiatica.

G Govindan1, T G Sambandan, M Govindan

  • 1Biomaterials Science and Engineering Laboratory, Massachusetts Institute of Technology, Cambridge, MA, USA.

Planta Medica
|May 24, 2007
PubMed
Summary
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A novel polyacetylene compound, cadiyenol, from Centella asiatica induces apoptosis in lymphoma cells and reduces nitric oxide production in macrophages without cytotoxicity.

Area of Science:

  • Natural Product Chemistry
  • Pharmacology
  • Cell Biology

Background:

  • Centella asiatica is a medicinal plant with various bioactive compounds.
  • Polyacetylene compounds are known for diverse biological activities.

Purpose of the Study:

  • To isolate and characterize a new polyacetylene compound from Centella asiatica.
  • To evaluate the cytotoxic and immunomodulatory effects of the isolated compound.

Main Methods:

  • Isolation and structural elucidation of the polyacetylene compound using spectroscopic methods.
  • Assessment of apoptosis induction in mouse lymphoma cells (P388D1).
  • Measurement of nitric oxide production in lipopolysaccharide-activated mouse macrophages.

Main Results:

Related Experiment Videos

  • A new polyacetylene compound, methyl 5-[(E)-9-hydroxy-1-(1-hydroxyhexyl)-2-methoxyundeca-3,10-diene-5,7-diynyloxy]pentanoate (cadiyenol), was identified.
  • Cadiyenol induced apoptosis in P388D1 cells (63% at 28 microM, IC50 = 24 +/- 2 microM) independently of the cell cycle.
  • Cadiyenol significantly reduced nitric oxide production in activated macrophages (70 +/- 2% at 24 microM) with no observable cytotoxicity.

Conclusions:

  • Cadiyenol is a novel bioactive polyacetylene isolated from Centella asiatica.
  • The compound exhibits potent anti-cancer properties by inducing apoptosis in lymphoma cells.
  • Cadiyenol possesses immunomodulatory effects, reducing nitric oxide production without causing cell death.