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A bioactive polyacetylene compound isolated from Centella asiatica.

G Govindan¹, T G Sambandan, M Govindan

¹Biomaterials Science and Engineering Laboratory, Massachusetts Institute of Technology, Cambridge, MA, USA.

|May 24, 2007

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A novel polyacetylene compound, cadiyenol, from Centella asiatica induces apoptosis in lymphoma cells and reduces nitric oxide production in macrophages without cytotoxicity.

Area of Science:

Natural Product Chemistry
Pharmacology
Cell Biology

Background:

Centella asiatica is a medicinal plant with various bioactive compounds.
Polyacetylene compounds are known for diverse biological activities.

Purpose of the Study:

To isolate and characterize a new polyacetylene compound from Centella asiatica.
To evaluate the cytotoxic and immunomodulatory effects of the isolated compound.

Main Methods:

Isolation and structural elucidation of the polyacetylene compound using spectroscopic methods.
Assessment of apoptosis induction in mouse lymphoma cells (P388D1).
Measurement of nitric oxide production in lipopolysaccharide-activated mouse macrophages.

Main Results:

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A new polyacetylene compound, methyl 5-[(E)-9-hydroxy-1-(1-hydroxyhexyl)-2-methoxyundeca-3,10-diene-5,7-diynyloxy]pentanoate (cadiyenol), was identified.
Cadiyenol induced apoptosis in P388D1 cells (63% at 28 microM, IC50 = 24 +/- 2 microM) independently of the cell cycle.
Cadiyenol significantly reduced nitric oxide production in activated macrophages (70 +/- 2% at 24 microM) with no observable cytotoxicity.

Conclusions:

Cadiyenol is a novel bioactive polyacetylene isolated from Centella asiatica.
The compound exhibits potent anti-cancer properties by inducing apoptosis in lymphoma cells.
Cadiyenol possesses immunomodulatory effects, reducing nitric oxide production without causing cell death.