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[Pharmacokinetics of triptolide in Beagle dogs]
Feng Shao1, Guang-Ji Wang, Jian-Guo Sun
1Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China.
Yao Xue Xue Bao = Acta Pharmaceutica Sinica
|May 25, 2007
Summary
This study developed a rapid LC-APCI/MS method to measure triptolide (TP) in dog plasma. Triptolide exhibits rapid elimination and high oral bioavailability in Beagle dogs.
Area of Science:
- Analytical Chemistry
- Pharmacokinetics
- Mass Spectrometry
Context:
- Triptolide (TP) is a compound with potential therapeutic applications.
- Accurate quantification of TP in biological matrices is crucial for pharmacokinetic studies.
- Existing methods may lack the sensitivity or speed required for comprehensive analysis.
Purpose:
- To develop and validate a sensitive and rapid liquid chromatography-atmospheric pressure chemical ionization/mass spectrometry (LC-APCI/MS) method for triptolide determination in dog plasma.
- To investigate the pharmacokinetic properties of triptolide in Beagle dogs following intravenous and oral administration.
Summary:
- A liquid-liquid extraction method using ethyl acetate was employed for sample preparation.
- The LC-APCI/MS method demonstrated linearity, precision (<10%), and accuracy (95.0%-105.0%) over a concentration range of 1-200 ng/mL.
- Pharmacokinetic studies in Beagle dogs revealed rapid elimination of triptolide, with a high absolute oral bioavailability of (75 ± 17)% at a dose of 0.05 mg/kg.
Impact:
- The validated LC-APCI/MS method provides a sensitive and rapid tool for triptolide quantification in plasma.
- The pharmacokinetic data elucidate triptolide's disposition in vivo, informing potential therapeutic development.
- Findings suggest triptolide's potential for oral drug delivery due to its high bioavailability.
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