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Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

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Related Experiment Video

Updated: Jul 14, 2026

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Embryonic stem cells.

Atindriya Biswas1, Robert Hutchins

  • 1Department of General Surgery, Royal London Hospital, London, UK. atruks@yahoo.co.uk

Stem Cells and Development
|May 25, 2007
PubMed
Summary

Embryonic stem (ES) cells can self-renew and differentiate, offering potential for human development studies and reparative therapies. Research focuses on optimizing culture conditions for these unique pluripotent cells.

Area of Science:

  • * Stem cell biology
  • * Developmental biology
  • * Regenerative medicine

Background:

  • * Embryonic stem (ES) cells possess unique self-renewal and differentiation capabilities.
  • * Long-term cultures of self-renewing ES cells are established in mice, monkeys, and humans.
  • * Maintaining the undifferentiated state of ES cells requires specific culture conditions like feeder cells, serum, or cytokines.

Purpose of the Study:

  • * To explore the manipulation of growth and differentiation conditions for stem cells.
  • * To leverage insights from mouse development studies for human stem cell research.
  • * To investigate the directed differentiation of ES cells toward specific lineages.

Main Methods:

  • * Culturing of embryonic stem cells under various conditions.

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Derivation of Human Embryonic Stem Cells by Immunosurgery
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Derivation of Human Embryonic Stem Cells by Immunosurgery

Published on: December 13, 2007

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
08:56

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Published on: August 1, 2010

Related Experiment Videos

Last Updated: Jul 14, 2026

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Derivation of Human Embryonic Stem Cells by Immunosurgery
11:56

Derivation of Human Embryonic Stem Cells by Immunosurgery

Published on: December 13, 2007

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
08:56

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Published on: August 1, 2010

  • * Studying developmental processes in model organisms like mice.
  • * Analyzing the pluripotency and differentiation potential of human ES cells.

    • Main Results:

    • * Emerging data show ES cells can be directed into lineage-specific differentiation pathways.
    • * Advances in culturing techniques enhance the exploitation of ES cell pluripotency.
    • * Mouse development studies inform the manipulation of human stem cells in vitro.

    Conclusions:

    • * Human ES cells offer a valuable system for studying human development mechanisms.
    • * Successful manipulation of ES cell pluripotency fuels hope for future stem cell-based therapies.
    • * Continued research into ES cell culture and differentiation is crucial for therapeutic applications.