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Published on: October 31, 2013
Characterization of two temperature-inducible promoters newly isolated from B. subtilis
Wang Li1, Heng-Xin Li, Sheng-Yue Ji
1College of Animal Sciences, Northwest A&F University, Yangling 712100, People's Republic of China.
Abstract:
Here, two temperature sensitive promoters, P2 and P7, isolated from Bacillus subtilis, were characterized. The production of beta-galactosidase driven by these promoters was much higher at 45 degrees C than that at 37 degrees C both in Escherichia coli and B. subtilis and that the P2 promoter showed higher expression strength in B. subtilis at 45 degrees C. Thereby, an efficient temperature-inducible expression system was constructed by using P2 promoter in B. subtilis. Thus, we isolated and characterized a newly temperature inducible promoter and exploited it as a potential expression element in B. subtilis.
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